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coloc plugin  (Oxford Instruments)


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    Structured Review

    Oxford Instruments coloc plugin
    Coloc Plugin, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coloc+plugin/Imaris/pmc12332193-309-15-18
    Average 99 stars, based on 44266 article reviews
    coloc plugin - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: The organization of submodality-specific touch afferent inputs in the vibrissa column
    Article Snippet: By using “Coloc plugin” of Imaris software, first, vGluT1 and Chodl PLAP double positive sites, which represented lanceolate-ending presynaptic termini, were extracted from all z-stack images.

    Article Title: The organization of submodality-specific touch afferent inputs in the vibrissa column.
    Article Snippet: By using ‘‘Coloc plugin’’ of Imaris software, first, vGluT1 and ChodlPLAP double-positive sites, which represented lanceolate-ending presynaptic termini, were extracted from all z stack images.

    Software:

    Article Title: Protocol to study internalization and localization dynamics of exogenously added proteins in detergent-permeabilized human cells
    Article Snippet: .. Note: The coefficient of co-localization between the two proteins can also be measured using the Coloc plugin of Imaris 7 software. ..

    Article Title: Impaired Presynaptic Function Contributes Significantly to the Pathology of Glycine Receptor Autoantibodies
    Article Snippet: .. For colocalization analysis of SIM images, the Coloc plugin of the software Imaris 8.4.1 was used. ..

    Article Title: Impaired Presynaptic Function Contributes Significantly to the Pathology of Glycine Receptor Autoantibodies
    Article Snippet: .. For colocalization analysis of SIM images, the Coloc plugin of the software Imaris 8.4.1 was used. ..



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    coloc plugin  (Oxford Instruments)
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    Coloc Plugin, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS <t>coloc</t> <t>plugin.</t> (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS <t>coloc</t> <t>plugin.</t> (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    plugin named colocalization spots  (Oxford Instruments)
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    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS <t>coloc</t> <t>plugin.</t> (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS <t>coloc</t> <t>plugin.</t> (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS coloc plugin. (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy

    doi: 10.1016/j.mtbio.2024.101225

    Figure Lengend Snippet: Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS coloc plugin. (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A).

    Techniques: Immunofluorescence, Staining, Software

    Sec22b and TRIM16 are involved in autophagy-mediated IL-1β secretion. (A) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for TRIM16 (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A). Gray arrows indicate the region of red-green overlap. (E) Pearson's colocalization coefficient for TRIM16 and LC3. (F) Immunofluorescence staining and 3D reconstruction for Sec22b (purple), LC3 (green), and DAPI (blue) (scale bar: 5 μm). White spots indicate co-localization. (G) 3D co-localization analysis conducted by IMARIS coloc plugin. (H) Co-localization analysis of purple channel (TRIM16) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of purple-green overlap. (J) Pearson's colocalization coefficient for Sec22b and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (*** indicates P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy

    doi: 10.1016/j.mtbio.2024.101225

    Figure Lengend Snippet: Sec22b and TRIM16 are involved in autophagy-mediated IL-1β secretion. (A) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for TRIM16 (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A). Gray arrows indicate the region of red-green overlap. (E) Pearson's colocalization coefficient for TRIM16 and LC3. (F) Immunofluorescence staining and 3D reconstruction for Sec22b (purple), LC3 (green), and DAPI (blue) (scale bar: 5 μm). White spots indicate co-localization. (G) 3D co-localization analysis conducted by IMARIS coloc plugin. (H) Co-localization analysis of purple channel (TRIM16) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of purple-green overlap. (J) Pearson's colocalization coefficient for Sec22b and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (*** indicates P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A).

    Techniques: Immunofluorescence, Staining, Software